Translational_Unit

Part:BBa_K3288061:Design

Designed by: Wooseok Kim   Group: iGEM19_SIS_Korea   (2019-10-16)


light inducible expression of signal peptide (NSP4) fused MHETase(18-604;WT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
    Illegal BamHI site found at 4016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI site found at 3480
    Illegal BsaI site found at 4284
    Illegal BsaI.rc site found at 525


Design Notes

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Source

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References